diff --git a/CHANGELOG.md b/CHANGELOG.md
index dba0f4dcdf..56a7be44b1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,6 +31,7 @@
 ### Fixes
 
 - Fix crash when unregistering `SystemEventsBroadcastReceiver` with try-catch block. ([#5106](https://github.com/getsentry/sentry-java/pull/5106))
+- Trim DSN string before parsing to avoid `URISyntaxException` caused by trailing whitespace ([#5113](https://github.com/getsentry/sentry-java/pull/5113))
 
 ### Dependencies
 
diff --git a/sentry/src/main/java/io/sentry/Dsn.java b/sentry/src/main/java/io/sentry/Dsn.java
index 836e2c5546..705d383266 100644
--- a/sentry/src/main/java/io/sentry/Dsn.java
+++ b/sentry/src/main/java/io/sentry/Dsn.java
@@ -50,8 +50,11 @@ URI getSentryUri() {
 
   Dsn(@Nullable String dsn) throws IllegalArgumentException {
     try {
-      Objects.requireNonNull(dsn, "The DSN is required.");
-      final URI uri = new URI(dsn).normalize();
+      final String dsnString = Objects.requireNonNull(dsn, "The DSN is required.").trim();
+      if (dsnString.isEmpty()) {
+        throw new IllegalArgumentException("The DSN is empty.");
+      }
+      final URI uri = new URI(dsnString).normalize();
       final String scheme = uri.getScheme();
       if (!("http".equalsIgnoreCase(scheme) || "https".equalsIgnoreCase(scheme))) {
         throw new IllegalArgumentException("Invalid DSN scheme: " + scheme);
diff --git a/sentry/src/main/java/io/sentry/SentryOptions.java b/sentry/src/main/java/io/sentry/SentryOptions.java
index 14f4acf51f..298e37795b 100644
--- a/sentry/src/main/java/io/sentry/SentryOptions.java
+++ b/sentry/src/main/java/io/sentry/SentryOptions.java
@@ -749,7 +749,7 @@ Dsn retrieveParsedDsn() throws IllegalArgumentException {
    * @param dsn the DSN
    */
   public void setDsn(final @Nullable String dsn) {
-    this.dsn = dsn;
+    this.dsn = dsn != null ? dsn.trim() : null;
     this.parsedDsn.resetValue();
 
     dsnHash = StringUtils.calculateStringHash(this.dsn, logger);
diff --git a/sentry/src/test/java/io/sentry/DsnTest.kt b/sentry/src/test/java/io/sentry/DsnTest.kt
index eaa129c207..6c454ad5c7 100644
--- a/sentry/src/test/java/io/sentry/DsnTest.kt
+++ b/sentry/src/test/java/io/sentry/DsnTest.kt
@@ -89,6 +89,24 @@ class DsnTest {
     assertEquals("http://host/api/id", dsn.sentryUri.toURL().toString())
   }
 
+  @Test
+  fun `dsn parsed with leading and trailing whitespace`() {
+    val dsn = Dsn("  https://key@host/id  ")
+    assertEquals("https://host/api/id", dsn.sentryUri.toURL().toString())
+  }
+
+  @Test
+  fun `when dsn is empty, throws exception`() {
+    val ex = assertFailsWith<IllegalArgumentException> { Dsn("") }
+    assertEquals("java.lang.IllegalArgumentException: The DSN is empty.", ex.message)
+  }
+
+  @Test
+  fun `when dsn is only whitespace, throws exception`() {
+    val ex = assertFailsWith<IllegalArgumentException> { Dsn("   ") }
+    assertEquals("java.lang.IllegalArgumentException: The DSN is empty.", ex.message)
+  }
+
   @Test
   fun `non http protocols are not accepted`() {
     assertFailsWith<IllegalArgumentException> { Dsn("ftp://publicKey:secretKey@host/path/id") }
diff --git a/sentry/src/test/java/io/sentry/SentryOptionsTest.kt b/sentry/src/test/java/io/sentry/SentryOptionsTest.kt
index 6868b55c2b..80510db931 100644
--- a/sentry/src/test/java/io/sentry/SentryOptionsTest.kt
+++ b/sentry/src/test/java/io/sentry/SentryOptionsTest.kt
@@ -588,6 +588,24 @@ class SentryOptionsTest {
     assertFalse(cacheDirPathWithoutDsn.contains(hash.toString()))
   }
 
+  @Test
+  fun `when setting dsn with whitespace, it is trimmed and produces the same cache dir path`() {
+    val dsn = "http://key@localhost/proj"
+    val options1 =
+      SentryOptions().apply {
+        setDsn(dsn)
+        cacheDirPath = "${File.separator}test"
+      }
+    val options2 =
+      SentryOptions().apply {
+        setDsn("  $dsn  ")
+        cacheDirPath = "${File.separator}test"
+      }
+
+    assertEquals(dsn, options2.dsn)
+    assertEquals(options1.cacheDirPath, options2.cacheDirPath)
+  }
+
   @Test
   fun `when options are initialized, idleTimeout is 3000`() {
     assertEquals(3000L, SentryOptions().idleTimeout)